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the mouse serpina3n cdna was subcloned into a 3xfg vector from sigma to generate 3xfg-s3n that was then transfected into hek293 cells.

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a mouse serpina3n cdna was subcloned into a 3x-flag vector 3xfg-s3n for transfection and flag detection, which again showed that serpina3n can also be detected in the intracellular compartment lysate and extracellular media of hek293 cells supplementary material, fig.
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We also subjected these mice to forced treadmill running to assess whether the observed improvement in histopathology conferred by the muscle-specific serpina3n transgene correlated with a functional benefit.

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